| 別品名 |
Stress-induced-phosphoprotein 1;STI1;Hsc70/Hsp90-organizing protein;Hop;Renal carcinoma antigen NY-REN-11;Transformation-sensitive protein IEF SSP 3521;STIP1;
|
| 種由来 |
Human
|
| 標識物 |
iFluorTM 647
|
| 精製度 |
Affinity Purified
|
| 適用 |
Western Blot
|
| 免疫動物 |
Rabbit
|
| 交差種 |
Human
Mouse
Rat
|
| GENE ID |
10963
|
| Accession No.(Gene/Protein) |
P31948
|
| Gene Symbol |
STIP1
|
| 感度 |
<20pg/ml
|
| 分子量 |
62639 MW
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| 形状 |
凍結乾燥品
|
| 参考文献 |
1. Chen, S., Smith, D. F. Hop as an adaptor in the heat shock protein 70 (Hsp70) and Hsp90 chaperone machinery. J. Biol. Chem. 273: 35194-35200, 1998.
2. Song, Y., Masison, D. C. Independent regulation of Hsp70 and Hsp90 chaperones by Hsp70/Hsp90-organizing protein Sti1 (Hop1).J. Biol. Chem. 280: 34178-34185, 2005.
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※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1. Western blot analysis of STIP1 using anti-STIP1 antibody (PB9896).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human COLO320 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: rat RH35 whole cell lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates,
Lane 9: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STIP1 antigen affinity purified polyclonal antibody (Catalog # PB9896) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STIP1 at approximately 63 kDa. The expected band size for STIP1 is at 63 kDa.
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|
|
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Figure 1. Western blot analysis of STIP1 using anti-STIP1 antibody (PB9896).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human COLO320 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: rat RH35 whole cell lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates,
Lane 9: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STIP1 antigen affinity purified polyclonal antibody (Catalog # PB9896) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STIP1 at approximately 63 kDa. The expected band size for STIP1 is at 63 kDa.
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|
| メーカー |
品番 |
包装 |
|
BBT
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PB9896-IFLUOR647
|
100 UG
|
※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
1週間程度
|
| 保存温度 |
-２０℃
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