| 別品名 |
Brain-derived neurotrophic factor; BDNF; Abrineurin
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| 種由来 |
Human
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| 交差種 |
Human
Mouse
Rat
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| 適用 |
Enzyme Linked Immunosorbent Assay
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| Accession No.(Gene/Protein) |
P23560
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| 適用サンプル |
Cell lysate
Culture supernatant
Plasma_citrate
Serum
Tissue homogenate
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| 測定範囲 |
Mature BDNF: 7.8 - 500 pg/mL; proBDNF: 15.6 - 1,000 pg/mL
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| 概要 |
The Biosensis Mature BDNF/proBDNF Combo RapidTM enzyme-linked immunosorbent assay (ELISA) Kit combines individual, but complementary ELISA kits for the two most important BDNF isoforms: Mature BDNF (BEK-2211) and full-length proBDNF (BEK-2217).
Both kits are sandwich ELISAs, useful for the quantification of mature BDNF and proBDNF in less than 4 hours in cell culture supernatants, serum, plasma (citrate), cell lysates and brain extracts only if used as directed, with a simplified protocol and no loss of sensitivity or specificity. Please refer to the most current kit protocols for BEK-2211 (Mature BDNF RapidTM ELISA) and BEK-2217 (proBDNF RapidTM ELISA), for specific use instructions for each substrate application, in particular blood samples. For human blood samples, we suggest the use of BEK-2241 (Mature BDNF/proBDNF Combo kit).
The Mature BDNF ELISA kit consists of a pre-coated mouse monoclonal anti-mature BDNF capture antibody, a biotinylated anti-mature BDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The proBDNF ELISA kit consists of a pre-coated polyclonal anti-proBDNF capture antibody, a biotinylated anti-mature BDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of mature BDNF or proBDNF present in samples and protein standards. A BDNF and proBDNF positive control (QC sample) is provided to assure consistent assay performance.
Both ELISA kits contain high quality protein calibrators. The Mature BDNF ELISA kit employs a recombinant E.coli-derived human mature BDNF standard approved by the World Health Organization (WHO, www.nibsc.org). The amino acid sequence of mature BDNF is identical for human, mouse, rat and a number of other species. This kit therefore is suitable to measure mature BDNF in all these species and uses the same antibodies and antigen. The proBDNF ELISA kit employs a recombinant mammalian, cleavage-resistant human proBDNF standard produced by Biosensis and validated against externally available proBDNF proteins. Due to a high degree of amino acid sequence homology, mouse and r
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| メーカー |
品番 |
包装 |
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BIE
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BEK-2240-SET
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2*1 PLATE
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
2週間程度
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| 法規制 |
安
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| 保存温度 |
４℃
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