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Immunofluorescence of A431 cells: PFA-fixed cells were stained with anti-B23 (60096-1-lg) labeled with FlexAble CoraLite Plus 594 Kit (KFA029, orange) and DAPI (blue). ？Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite Plus 750 Kit (KFA024) and anti-CD4 (clone RPA-T4, 65143-1-Ig) labeled with FlexAble CoraLite Plus 594 Kit (KFA029). Cells are not fixed.
Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite Plus 594 Kit (KFA029) and co-stained anti-CD4 (CL750-65134) antibody. Cells are not fixed.
Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-CD45RA (clone F8-11-13, 65226-1-Ig) labeled with FlexAble CoraLite Plus 594 Kit (KFA029) and co-stained anti-CD45RO (CL750-65150) antibody. Cells are not fixed.
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