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Immunofluorescence of A431 cells: PFA-fixed cells were stained with rat anti-Tubulin alpha antibody labeled with FlexAble CoraLite Plus 488 Kit (KFA121, green). Cell nuclei were stained with DAPI (blue). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Immunofluorescence of A431: Formaldehyde-fixed A431 cells were stained with rat IgG2a kappa anti-Tubulin antibody labeled with FlexAble CoraLite Plus 488 Kit (KFA121, green) and rat IgG1 kappa anti-RPA32 antibody labeled with FlexAble CoraLite Plus 555 Kit (KFA122, red). Epifluorescence images were acquired with a 20x objective and post-processed.
Immunofluorescence of A431: Formaldehyde-fixed A431 cells were stained with rat IgG2a kappa anti-Tubulin antibody labeled with FlexAble CoraLite Plus 488 Kit (KFA121, green) and rat IgG2b kappa anti-RNA PolymeraseII antibody labeled with FlexAble CoraLite Plus 555 Kit (KFA122, red). Epifluorescence images were acquired with a 20x objective and post-processed.
Human peripheral blood lymphocytes (PBMC) were either un-stimulated (left) or stimulated with PMA and Ionomycin for 6 hours in the presence of monensin (right). ？Cells were fixed and permeabilized with Foxp3/Transcription Factor Staining Buffer Kit (PF00011). After wash, cells were stained with rat anti-human TNF-α antibody labeled with FlexAble CoraLite Plus 488 for Rat Kappa Light Chain (KFA121) and CD3 antibody (CL750-65151).
1X10^6 of LPS treated mouse splenocytes were surface stained with rat anti-Mouse CD86 (65068-1-Ig, Clone: GL1, red) or rat IgG2a isotype control (65209-1-Ig, blue) labeled with FlexAble CoraLite Plus 488 for Rat Kappa Light Chain (KFA121). Cells were not fixed.
1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5μg rat anti-Mouse CD4 (65104-1-Ig, Clone: GK1.5) labeled with FlexAble CoraLite Plus 488 Kit (KFA121) and 0.5μg rat anti-Mouse CD3 (65077-1-Ig, Clone: 17A2) labeled with FlexAble CoraLite Plus 647 Kit (KFA123). Cells were not fixed.
Immunofluorescence of A431 cells: PFA-fixed cells were co-stained with rat anti-RNA Pol II Ser5 antibody labeled with FlexAble CoraLite Plus 647 Kit (KFA123, magenta) and with rat anti-Tubulin alpha antibody labeled with FlexAble CoraLite Plus 488 Kit (KFA121, green). Cell nuclei were stained with DAPI (blue). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
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