免疫動物 |
Alpaca
|
構成内容 |
● HA-Trap Magnetic Particles M-270: 20 rxns (500 ul slurry) ● Lysis buffer (10 mM Tris/Cl pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.5 % Nonidet P40 Substitute, 0.09 % sodium azide): 30 mL ● RIPA buffer (10 mM Tris/Cl pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.1 % SDS,1 % Triton X-100, 1 % Deoxycholate, 0.09 % sodium azide): 30 mL ● Wash buffer (10 mM Tris/Cl pH 7.5, 150 mM NaCl, 0.05 % Nonidet P40 Substitute, 0.5 mM EDTA, 0.018 % sodium azide): 50 mL (after dilution with 40 mL H2O) ● Dilution buffer (10 mM Tris/Cl pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.018 % sodium azide): 50 mL (after dilution with 40 mL H2O) ● Acidic elution buffer (200 mM glycine pH 2.5): 3x 1 mL
|
その他 |
[備考]旧Chromotek(クロモテック、ドイツ)社商品。2020年10月よりProteintech Group(プロテインテック、米国)社ブランドの一部になりました。
|
※サムネイル画像をクリックすると拡大画像が表示されます。
The HA-Trap Magnetic Particles M-270 Kit was used to immunoprecipitate TOM70-HA fusion protein from either untransfected (mock) HEK293T cells or HEK293T cell transfected with full-length TOM70-HA construct. SDS-PAGE analysis was done on samples from the Input (I), Flow-through (F), Bound (B) fractions.
WB detection of TOM70-HA fusion protein following immunoprecipitation with HA-Trap Magnetic Particles M-270 Kit from either untransfected (mock) HEK293T cells or HEK293T cells transfected with full-length TOM70-HA construct. Samples from the Input (I), Flow-Through (F), and Bound (B) fractions were used in the WB analysis. Detection was completed using TOM70 Monoclonal Antibody (66593-1-Ig) and Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM001).
|
|
The HA-Trap Magnetic Particles M-270 Kit was used to immunoprecipitate TOM70-HA fusion protein from either untransfected (mock) HEK293T cells or HEK293T cell transfected with full-length TOM70-HA construct. SDS-PAGE analysis was done on samples from the Input (I), Flow-through (F), Bound (B) fractions.
|
|
|
メーカー |
品番 |
包装 |
PGI
|
ATDK-20
|
20 RXN
|
※表示価格について
当社在庫 |
なし
|
納期目安 |
1週間程度
|
法規制 |
化管
|
保存温度 |
4℃禁凍結
|
|