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Various lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-PSMB1 polyclonal antibody (11749-1-AP) at dilution of 1:10000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) was used at 1:20000 for detection.
Various lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-Beta Catenin polyclonal antibody (51067-2-AP) at dilution of 1:50000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) was used at 1:20000 for detection.
Various lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-TOM70 polyclonal antibody (14528-1-AP) at dilution of 1:10000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) was used at 1:20000 for detection.
Various lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-TDP43 recombinant antibody (80001-1-RR) at dilution of 1:20000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) was used at 1:20000 for detection.
PC-3 and Calyculin A treated PC-3 cell lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-Phospho-EIF4B (Ser406) Recombinant antibody (80059-1-RR) at dilution of 1:20000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001), leading competitor’s non cross-adsorbed and cross-adsorbed secondary antibodies were all used at 0.05ug/mL for detection.
HEK-293 and HepG2 cell lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-ZO-1 polyclonal antibody (21773-1-AP) at dilution of 1:50000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001), leading competitor’s non cross-adsorbed and cross-adsorbed secondary antibodies were all used at 0.05ug/mL for detection.
Various lysates were subjected to SDS-PAGE followed by western blot with Beta tubulin rabbit recombinant antibody (80713-1-RR) at dilution of 1:20000. Two batches of Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) were used at 1:20000 for detection.
Rabbit total IgG, Mouse total IgG, Rat total IgG, Pig total IgG, Human total IgG, Bovine total IgG were coated at 100 ng/well. 0.125 ug/mL Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) was used for detection. The result indicates that RGAM001 is highly specific for rabbit IgG and does not react with other species tested in the experiment.
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