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Immunofluorescence of MCF-7 cells: MCF-7 cells were fixed with 4% PFA and stained with Rabbit anti-ZO1 polyclonal antibody (21773-1-AP, 1:2000, green) and mouse anti-Alpha Tubulin monoclonal antibody (66031-1-Ig, 1:1000, magenta). Multi-rAb CoraLite Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002, 1:500) and Multi-rAb CoraLite Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005, 1:500) were used for detection.
Immunofluorescence of Hela cells: Hela cells were fixed with 4% PFA and stained with Rabbit anti-Alpha Tubulin polyclonal antibody (11224-1-AP, 1:200, green) and mouse anti-NPM1 monoclonal antibody (60096-1-Ig, 1:1000, magenta). Multi-rAb CoraLite Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002, 1:500) and Multi-rAb CoraLite Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005, 1:500) were used for detection.
Immunofluorescence of mouse cerebellum: mouse cerebellum FFPE section was stained with Rabbit anti-NeuN polyclonal antibody (26975-1-AP, 1:200, green) and mouse anti-Calbindin-D28k monoclonal antibody (66394-1-Ig, 1:200, magenta). Multi-rAb CoraLite Plus 488 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR002, 1:500) and Multi-rAb CoraLite Plus 647 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM005, 1:500) .
Immunofluorescence of rat brain: rat brain FFPE section was stained with Rabbit anti-GFAP polyclonal antibody (16825-1-AP, 1:200, green) and mouse anti-NeuN monoclonal antibody (66836-1-Ig, magenta). Multi-rAb CoraLite Plus 488 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR002, 1:500) and Multi-rAb CoraLite Plus 647 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM005, 1:500) .
1X10^6 MOLT4 were surface stained with 0.2 ug Anti-Human CD8 (65204-1-Ig, Clone: UCHT4) and Mouse IgG2a Isotype Control 66360-3-Ig. Multi-rAb CoraLite Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM005 was used at 1:500 for detection.
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Immunofluorescence of MCF-7 cells: MCF-7 cells were fixed with 4% PFA and stained with Rabbit anti-ZO1 polyclonal antibody (21773-1-AP, 1:2000, green) and mouse anti-Alpha Tubulin monoclonal antibody (66031-1-Ig, 1:1000, magenta). Multi-rAb CoraLite Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002, 1:500) and Multi-rAb CoraLite Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005, 1:500) were used for detection.
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| 別包装 |
あり
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| 種由来 |
Mouse
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| 標識物 |
CoraLite(R) 647
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| 精製度 |
Ig fraction - Protein G
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| 適用 |
Immuno Fluorescence
Flow Cytometry
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| 免疫動物 |
Goat
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| 交差種 |
Mouse
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| 形状 |
液状
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| その他 |
[Buffer]PBS with 50% glycerol, 10 mg/mL BSA, 0.1% Proclin-300, pH 7.4.
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| メーカー |
品番 |
包装 |
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PGI
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RGAM005
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200 UL
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