Anti IgG (H+L), Multi-rAb, Recombinant Secondary Antibody

Immunofluorescence of MCF-7 cells: MCF-7 cells were fixed with 4% PFA and stained with Rabbit anti-ZO1 polyclonal antibody (21773-1-AP, 1:2000, green) and mouse anti-Alpha Tubulin monoclonal antibody (66031-1-Ig, 1:1000, orange). Multi-rAb CoraLite Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002, 1:500) and Multi-rAb CoraLite Plus 555-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM003, 1:500) were used for detection.

Immunofluorescence of Hela cells: Hela cells were fixed with 4% PFA and stained with Rabbit anti-Alpha Tubulin polyclonal antibody (11224-1-AP, 1:200, green) and mouse anti-NPM1 monoclonal antibody (60096-1-Ig, 1:1000, orange). Multi-rAb CoraLite Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002, 1:500) and Multi-rAb CoraLite Plus 555-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM003, 1:500) were used for detection.

Immunofluorescence of mouse cerebellum: mouse cerebellum FFPE section was stained with Rabbit anti-NeuN polyclonal antibody (26975-1-AP, 1:200, green) and mouse anti-Calbindin-D28k monoclonal antibody (66394-1-Ig, 1:200, orange). Multi-rAb CoraLite Plus 488 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR002, 1:500) and Multi-rAb CoraLite Plus 555 conjugated Recombinant Goat anti-mouse secondary antibody were used for detection (RGAM003, 1:500) .

Immunofluorescence of rat brain: rat brain FFPE section was stained with Rabbit anti-GFAP polyclonal antibody (16825-1-AP, 1:200, green) and mouse anti-NeuN monoclonal antibody (66836-1-Ig, orange). Multi-rAb CoraLite Plus 488 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR002, 1:500) and Multi-rAb CoraLite Plus 555 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM003, 1:500) .

TOM70 polyclonal antibody (14528-1-AP, 1:200) Multi-rAb CoraLite Plus 555 conjugated Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR003, 1:600) was used for detection. Nucleus was stained with DAPI (blue). The experiment was performed in Chromotek’s lab and image was recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

1X10^6 MOLT4 were surface stained with 0.2 ug Anti-Human CD8 (65204-1-Ig, Clone: UCHT4) and Mouse IgG2a Isotype Control 66360-3-Ig. Multi-rAb CoraLite Plus 550-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM003 was used at 1:500 for detection.