Anti FLAG^(R) DYKDDDDK  

Western blot of Peroxidase conjugated Rabbit Anti-DYKDDDDK same epitope as Sigma's Anti-FLAG antibody. Lane 1: BAP FLAG C-Term. Lane 2: BAP FLAG N-Term. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 49 kDa, 49 kDa for BAP FLAG. Other band(s): None.

Western Blot of Peroxidase conjugated Rabbit Anti-DYKDDDDK same epitope as Sigma's Anti-FLAG antibody. Lane 1: Opal Presatained Molecular Weight Marker (p/n MB-210-0500). Lane 2: FLAG Positive Control Lysate (p/n MB- ) Reduced 5μg [+]. Lane 3: 12 Epitope Tag Lysate-GST (p/n MB- ) Reduced 5μg [+]. Lane 4: MBP (p/n ) Reduced 0.1μg [-]. Lane 5: rGST (p/n ) Reduced 0.1μg [-]. Primary Antibody: Antibody for the detection of FLAG？ HRP 1.0μg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP MX10 (p/n 611-103-122) at 1:70,000 for 30 mins at RT.Block: Universal Block Out Buffer (p/n MB-073) 1hr RT.Exposure 1sec.