Anti Lac I  

Western Blot of Rabbit Anti-Lac I Antibody. Lane 1: Molecular Weight Marker (3μL). Lane 2: recombinant LAC-I (0.05μg). Primary Antibody: Anti-LAC-I at 1:1000 for 1hr at RT. Secondary Antibody: Goat anti-Rabbit IgG HRP (p/n 611-103-122) at 1:40,000 for 30mins at RT. Block: BlockOut Buffer (p/n MB-073) for 30mins at RT. Predicted/Observed MW: ~38kDa.

Western blot using Rockland's affinity purified anti-Lac I antibody shows detection of a 38 kDa band corresponding to recombinant Lac I (arrowhead). The blot was blocked with 5% BLOTTO (p/n B501-0500) in TTBS overnight at 4°C. Primary antibody was used at a 1:1,000 dilution followed by reaction with a 1:10,000 dilution of HRP goat anti-rabbit IgG. ECL was used for detection. Personal communication, S. Hughes & M. Abram, NCI, Bethesda, MD.