別品名 |
PD-1 Antibody: PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1, PD1, Programmed cell death protein 1, Protein PD-1, PDCD1, PDCD-1
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抗原部位 |
C-terminus
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot IHC paraffin embedding section Enzyme Linked Immunosorbent Assay Immuno Fluorescence
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免疫動物 |
Rabbit
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抗体クラス |
IgG
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交差種 |
Human Mouse
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GENE ID |
5133
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Accession No.(Gene/Protein) |
Q15116
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Gene Symbol |
PDCD1
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形状 |
液状
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推奨品 |
ポジティブコントロール 品番:1208 - THP-1 Cell Lysate
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その他 |
[Protein GI Number]145559515 [Swiss-Prot No]Q15116
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参考文献 |
Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90. Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95. Zhong X, Bai C, Gao W, et al. Suppression of expression and function of negative immune regulator PD-1 by certain pattern recognition and cytokine receptor signals associated with immune system danger. Int. Immunol. 2004; 16:1181-8. Nishimura H, Nose M, Hiai H, et al. Development of lupus-like autoimmune diseases by the disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor. Immunity 1999; 11:141-51.
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Figure 1 KO Validation in HeLa Cells Loading: 10 μg of HeLa WT cell lysates or PD-1 KO cell lysates. Antibodies: PD-1, 4065 (4 μg/mL) and beta-actin 3779 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 2 KD Validation in HeLa Cells Loading: 10 μg of HeLa WT cell lysates or PD-1 KD cell lysates. Antibodies: PD-1, 4065 (4 μg/mL) and beta-actin 3779 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 3 Western Blot Validation in Human and Mouse Cell Lines Loading: 15 μg of lysates per lane.Antibodies: PD-1 4065 (4 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 4 Western Blot Validation in THP-1 Cell Lysate in the (A) absence and (B) presence of blocking peptide Loading: 15 μg of lysates per lane.Antibodies: PD-1, 4065 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 5 Western Blot Validation in Rat Thymus Cell Lysate Loading: 15 μg of lysates per lane.Antibodies: PD-1 4065 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 6 Immunohistochemistry Validation of PD-1 in Human Tonsil Tissue Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PD-1 antibody (4065) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4℃. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 6 Immunohistochemistry Validation of PD-1 in Human Tonsil Tissue Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PD-1 antibody (4065) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4℃. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 8 Immunohistochemistry Validation of PD-1 in Human Brain Tissue Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-PD-1 antibody (4065) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4℃. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Figure 1 KO Validation in HeLa Cells Loading: 10 μg of HeLa WT cell lysates or PD-1 KO cell lysates. Antibodies: PD-1, 4065 (4 μg/mL) and beta-actin 3779 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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メーカー |
品番 |
包装 |
PSC
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4065
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100 UG [1 mg/mL]
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※表示価格について
当社在庫 |
なし
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納期目安 |
3週間程度
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保存温度 |
-20℃
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