別品名 |
PD-L1 Antibody: B7-H, B7H1, PDL1, PD-L1, PDCD1L1, PDCD1LG1, Programmed cell death 1 ligand 1, B7 homolog 1
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot IHC paraffin embedding section Enzyme Linked Immunosorbent Assay Immuno Fluorescence Flow Cytometry
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免疫動物 |
Rabbit
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抗体クラス |
IgG
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交差種 |
Human Mouse Rat
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GENE ID |
29126
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Accession No.(Gene/Protein) |
NP_054862
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Gene Symbol |
CD274
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形状 |
液状
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推奨品 |
ポジティブコントロール 品番:1207 - Raji Cell Lysate ポジティブコントロール 品番:1301 - Human Heart Tissue Lysate
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その他 |
[Protein GI Number]7661534 [Swiss-Prot No]Q9NZQ7
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参考文献 |
Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90. Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95. LaGier J and Pober JS. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 2006; 67:568-78.
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Figure 1 Western Blot Validation of PD-L1 in HeLa Cells Loading: 15 μg of lysates per lane.Antibodies: 4059 (A: 1 μg/mL, B: 2 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Human and Mouse cell lines Loading: 15 μg of lysates per lane.Antibodies: 4059 (2 μg/mL), RF16035 (2 μg/mL), and beta-actin (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit and or anti-mouse IgG HRP conjugate at 1:10000 and 1:5000 dilution, respectively.
Figure 3 Validation with PD-L1 siRNA Knockdown in HeLa Cells HeLa cells were transfected with control siRNAs (lane 1) or PD-L1 siRNAs (lane 2) Loading: 10 μg of HeLa whole cell lysates per lane.Antibodies: 4059 (2 μg/mL) and GAPDH (3783, 0.02 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
Figure 4 Validation with PD-L1 overexpression in 293 cells Loading: Lysates/proteins at 15 μg per lane.Lane 1: non-transfected 293 cellsLane 2: PD-L1 overexpressed 293 cellsAntibodies: 4059 (1 μg/mL). 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 5 Immunohistochemistry Validation of PD-L1 in Human Tonsil Cells Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PD-L1 antibody (4059) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4℃. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 6 Immunofluorescence Validation of PD-L1 in Human Heart Immunofluorescent analysis of 4% paraformaldehyde-fixed human heart tissue labeling PD-L1 with 4059 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on human heart tissue.
Figure 7 Flow Cytometry Validation of PD-L1 Overlay histogram showing A-20 cells stained with 4059 (red line, 1μg/1x106 cells). 1 h incubation at 4℃ in 2% FBS/PBS. Followed by secondary antibody 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 1 h 4℃. Isotype control antibody (Green line) was mouse IgG1 (1μg/1x106 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Figure 8 Immunohistochemistry Validation of PD-L1 in Rat Heart Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-PD-L1 antibody (4059) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4℃. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 9 Immunohistochemistry Validation of PD-L1 in Human Heart Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-PD-L1 antibody (4059) at 2.5 μkg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4℃. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 10 Immunofluorescence Validation of PD-L1 in Rat Heart Immunofluorescence analysis of 4% paraformaldehyde-fixed rat heart tissue labeling PD-L1 with 4059 at 20 μg/ml, followed by goat anti-rabbit IgG secondary antibody at 1/250 dilution (red).
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Figure 1 Western Blot Validation of PD-L1 in HeLa Cells Loading: 15 μg of lysates per lane.Antibodies: 4059 (A: 1 μg/mL, B: 2 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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メーカー |
品番 |
包装 |
PSC
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4059
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100 UG [1 mg/mL]
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※表示価格について
当社在庫 |
なし
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納期目安 |
3週間程度
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保存温度 |
-20℃
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