Anti AKT  

Various whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-Akt(PTR2314) antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody.Lane 1: NIH-3T3Lane 2: MCF7Lane 3: HepG2

Various whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-Akt(PTR2314) antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody.Lane 1: NIH-3T3Lane 2: MCF7Lane 3: HepG2Predicted band size: 56kDaObserved band size: 60kDa

Akt was immunoprecipitated from HEK293 whole cell lysate with anti-Akt antibody. Western blot was performed on the immunoprecipitate using anti-Akt antibody, and followed by the HRP-conjugated Goat anti-Mouse IgG(H + L) antibody.Lane 1: HEK293 whole cell lysateLane 2: anti-Akt antibody IP in HEK293 whole cell lysateLane 3: Mouse monoclonal IgG (MNH209) in HEK293 whole cell lysate.

Akt was immunoprecipitated from HEK293 whole cell lysate with anti-Akt antibody. Western blot was performed on the immunoprecipitate using anti-Akt antibody, and followed by the HRP-conjugated Goat anti-Mouse IgG(H + L) antibody.Lane 1: HEK293 whole cell lysateLane 2: anti-Akt antibody IP in HEK293 whole cell lysateLane 3: Mouse monoclonal IgG (MNH209) in HEK293 whole cell lysate.