Anti Active Caspase-3  

Immunofluorescence analysis of rat-lung tissue. 1,Active Caspase-3 Monoclonal Antibody(5E1)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Hela cell. 1,FoxO1 (phospho Ser256) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). Active Caspase-3 Monoclonal Antibody(5E1)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:SA0837 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:SA0662 was diluted at 1:1000(room temperature, 50min).

Immunohistochemical analysis of paraffin-embedded Human Tonsil Tissue using Active Caspase-3 Monoclonal Antibody.

Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,Active Caspase-3 Monoclonal Antibody(5E1) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,Active Caspase-3 Monoclonal Antibody(5E1) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,Active Caspase-3 Monoclonal Antibody(5E1) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Western blot analysis of 1) Hela, 2) 3T3, 3) Rat Brain Tissue using Active Caspase-3 Monoclonal Antibody.

Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000