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Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-CD147 antibody labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG1 (KFA027) and Streptavidin-ATTO594？ (yellow). Nuclei are stained with DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Flow Cyotometry of PBMC. 1X10^6 human PBMC were stained with anti-human CD86 antibody (65156-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG1 (KFA027) and Streptavidin-APC. The cells were co-stained with either mouse IgG2b isotype control or anti-human CD14 antibody (CL488-65246). Cells are not fixed, Monocytes are gated.
Flow cytometry of PBMC. 1X10^6 human PBMC were stained with anti-human CD86 antibody (65156-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG1 (KFA027) and Streptavidin-PE. The cells were co-stained with either mouse IgG2b isotype control or anti-human CD14 antibody (CL488-65246). Cells are not fixed, Monocytes are gated.
Flow cytometry of PBMC. 1X10^6 human PBMC were stained with anti-human CD86 antibody (65156-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG1 (KFA027) and Streptavidin-PE. The cells were co-stained with either mouse IgG2b isotype control or anti-human CD14 antibody (CL488-65246). Cells are not fixed, Monocytes are gated.
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using anti-Cytokeratin 5 antibody (66727-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG1 (KFA027) and used at a dilution of 1:400 (under 10x lens & 40xlens). Streptavidin Poly-HRP and DAB substrate was used for detection. Heat mediated antigen retrieval performed with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using anti-Cytokeratin 5 antibody (66727-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG1 (KFA027) and used at a dilution of 1:400 (under 10x lens & 40xlens). Streptavidin Poly-HRP and DAB substrate was used for detection. Heat mediated antigen retrieval performed with Tris-EDTA buffer (pH 9.0).
Flow Cyotometry of PBMC. 1X10^6 human PBMC were stained with anti-human CD86 antibody (65156-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG1 (KFA027) and Streptavidin-APC. The cells were co-stained with either mouse IgG2b isotype control or anti-human CD14 antibody (CL488-65246). Cells are not fixed, Monocytes are gated.
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