Anti Thyroid Hormone Receptor β1 (THRB1)

Western blot using Rockland's affinity purified anti-THRB1 antibody shows detection of in vitro translated THRB1 (lane 2) and human thyroid stable cell line expressing THTRb1 (lane 3). No staining is evident in non transfected human thyroid cells (lane 1), over expressed THRA (lane 4) or HeLa stable cell line expressing THRA1 (lane 5). The band at ~62 kDa, indicated by the arrowheads, corresponds to THRB. Personal communication, H. Ying, NCI, Bethesda, MD

Immunofluorescence microscopy anti -THRB1 (Thyroid hormone receptor Beta 1) antibody 600-41-A96. Tissue: Mouse Dendritic cells.Primary antibody: Anti THRB1 1:100 1 hr PBS 3% BSA (left) Normal rabbit IgG isotype control (right).Secondary Ab: 488 dye conjugate 1:1000 1 hr. Mounting: Fluoromount-G (Southern Biotechnology Associates, Birmingham, AL) for examination.This image appeared originally in Mascanfroni, Ivan D ; del Mar Montesinos M; Alamino Vanina A. ; Susperreguy S, Nicola JP, Ilarregui JM, Masini-Repiso AM, Rabinovich GA, Pellizas CG (2010) Nuclear Factor (NF)-kappa B-dependent Thyroid Hormone Receptor beta(1) Expression Controls Dendritic Cell Function via Akt Signaling. Journal of Biological Chemistry 285 (13), 9569-9582. DOI: 10.1074/jbc.M109.071241 Published: MAR 26 2010.Copyright ^(C) 2010, by the American Society for Biochemistry and Molecular Biology.