Anti STAT2, phospho Tyr690

Western blot using Rockland Immunochemicals affinity purified anti-Stat2pY690 antibody shows detection of Stat2pY690 protein (arrowhead). Loaded Jurkat cells treated without (left lane) or with (right lane) 1000U/mL of IFN-a for 15 min at 37C. Primary antibody was used at 1:1,000. Personal Communication, A.Gamero, NCI, Bethesda, MD.

Immunohistochemistry with anti-STAT2 pY690 antibody showing nuclear positivity in epithelial cells of mouse urinary bladder tissue at 20x and 4x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval and then incubated with rabbit anti-STAT2 pY690 antibody 600-41-A93 at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3'3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.