| 別品名 |
Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous Tubulin K-alpha-1, TUBA1B, tubulin loading control, Alpha-tubulin, Tubulin alpha-1A, TUBA1A, TUBA3, LIS3
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| 抗原部位 |
a.a.427-441
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| 種由来 |
Human
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immuno Fluorescence
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| 免疫動物 |
Rabbit
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| 交差種 |
Human
Mouse
Rat
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| Accession No.(Gene/Protein) |
P68363
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| Gene Symbol |
TUBA1B
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| 形状 |
滅菌済み液状品
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| 参考文献 |
[Pub Med ID]26352206, 26574648, 26972791, 28447619, 28882191, 29875444, 29963231, 30212902, 22037769, 22258250, 23575666, 23653213, 24004954, 24811749, 24840943, 29036638, 30651288, 31466866, 31722198, 33184056+他多数
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※サムネイル画像をクリックすると拡大画像が表示されます。
miR 155 and FOXP3 down regulate endogenous ZEB2 in human breast cancer cells resulting in altered levels of EMT markers Vimentin and E cadherin(A) Relative abundance of ZEB2 and ZEB1 protein in WT, GFP or FOXP3 overexpressing BT549 cells transfected with miR 155 or miR control. Relative abundance of protein was determined by quantitation of the abundance of ZEB2 or ZEB1 proteins normalised to reference protein α Tubulin by western blot analysis. Quantitation of bands was carried out using Image J software. Mean + SD plotted. Students t test ***P < 0.001. ZEB1 protein expression as above. n = 3 experiments. (B) ZEB2 and ZEB1 protein in WT, GFP or FOXP3 overexpressing BT549 cells transfected with miR 155 or miR control by western blot. Representative western blot shown. (C) Relative abundance of Vimentin and E cadherin protein in WT, GFP or FOXP3 overexpressing BT549 cells transfected with miR 155 or miR control. Relative abundance of protein was determined by quantitating the abundance of E cadherin or Vimentin proteins and normalising to reference protein B Actin by western blot analysis. Quantitation of bands was carried out using Image J software. Mean + SD plotted. Students t test ***P < 0.001, **P < 0.01. n = 3 experiments. (D) Vimentin and E cadherin protein in WT, GFP or FOXP3 overexpressing BT549 cells transfected with miR 155 or miR control analysed by western blot. Representative western blot shown. Figure provided by CiteAb. Source: Oncotarget, PMID: 29963231.
Immunofluorescence microscopy of Rabbit Anti-alpha-Tubulin antibody using HeLa cells fixed with PFA. Anti-alpha-Tubulin Antibody was used at 1 ug/mL, O/N at 4？C. Secondary antibody: Anti-RABBIT IgG DyLightTM 488 Conjugated Preadsorbed (p/n 611-741-127) at 2 ug/ml for 1 h at RT. Localization: TUBA1B is the major constituent of microtubules in the cytoplasm. Staining: Tubulin as green fluorescent signal with DAPI (blue) nuclear counterstain.
ELISA results of purified Rabbit anti-alpha-Tubulin Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n 611-103-122) and TMB ELISA Peroxidase Substrate (p/n TMBE-1000).
Western Blot of Rabbit Anti-Alpha Tubulin Antibody. Lane 1: whole cell lysates from mouse brain (p/n W10-000-T004). Lane 2: rat brain (p/n W12-000-T077). Lane 3: A431 cells (p/n W09-000-361). Lane 4: Jurkat cells (p/n W09-001-370). Lane 5: HeLa cells (p/n W09-000-364). Load: 35 ug per lane. Primary antibody: Alpha Tubulin antibody at 1:1,200 for overnight at 4C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO (p/n B501-0500) overnight at 4C. Predicted/Observed size: ~50 kDa corresponding to alpha tubulin (arrowhead). Other band(s): none.
Western Blot of Rabbit anti-alpha-Tubulin antibody. Lane 1: HeLa WCL (p/n W09-000-364). Lane 2: NIH/3T3 WCL (p/n W10-000-358). Load: 10 ug per lane. Primary antibody: alpha-Tubulin antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) for 30 min at RT. Predicted/Observed size: 50 kDa, 50 kDa for alpha-Tubulin. Other band(s): N/A.
Western Blot of Rabbit anti-Alpha-Tubulin antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: HEK293 lysate (p/n W09-000-365). Lane 2: HeLa Lysate (p/n W09-000-364). Lane 3: MCF-7 Lysate (p/n W09-000-360). Lane 4: Jurkat Lysate (p/n W09-000-370). Lane 5: A431 Lysate (p/n W09-000-361). Lane 6: LNCaP Lysate (p/n W09-001-GJ9). Lane 7: A-172 Lysate (p/n W09-001-GL5). Lane 8: NIH/3T3 Lysate (p/n W10-000-358). Load: 35 ug per lane. Primary antibody: Alpha-Tubulin antibody at 1:2,000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS (p/n MB-082) for 30 min at RT. Predicted/Observed size: 50 kDa for Alpha-tubulin.
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miR 155 and FOXP3 down regulate endogenous ZEB2 in human breast cancer cells resulting in altered levels of EMT markers Vimentin and E cadherin(A) Relative abundance of ZEB2 and ZEB1 protein in WT, GFP or FOXP3 overexpressing BT549 cells transfected with miR 155 or miR control. Relative abundance of protein was determined by quantitation of the abundance of ZEB2 or ZEB1 proteins normalised to reference protein α Tubulin by western blot analysis. Quantitation of bands was carried out using Image J software. Mean + SD plotted. Students t test ***P < 0.001. ZEB1 protein expression as above. n = 3 experiments. (B) ZEB2 and ZEB1 protein in WT, GFP or FOXP3 overexpressing BT549 cells transfected with miR 155 or miR control by western blot. Representative western blot shown. (C) Relative abundance of Vimentin and E cadherin protein in WT, GFP or FOXP3 overexpressing BT549 cells transfected with miR 155 or miR control. Relative abundance of protein was determined by quantitating the abundance of E cadherin or Vimentin proteins and normalising to reference protein B Actin by western blot analysis. Quantitation of bands was carried out using Image J software. Mean + SD plotted. Students t test ***P < 0.001, **P < 0.01. n = 3 experiments. (D) Vimentin and E cadherin protein in WT, GFP or FOXP3 overexpressing BT549 cells transfected with miR 155 or miR control analysed by western blot. Representative western blot shown. Figure provided by CiteAb. Source: Oncotarget, PMID: 29963231.
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| メーカー |
品番 |
包装 |
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RKL
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600-401-880
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200 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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