Anti beta Tubulin  

All lanes : Anti-beta Tubulin at 1:2000 dilution
Lane 1: C2C12 whole cell lysate
Lane 2: L6 whole cell lysate
Lane 3: mouse brain lysate
Lane 4: mouse spleen whole cell lysate
Lane 5: mouse thymus lysate
Lane 6: NIH/3T3 whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 50 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

M32657-1 staining beta Tubulin in mouse thymus tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Overlay histogram showing C2C12 cells stained with M32657-1 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M32657-1, 1:25 dilution) for 60 min at 37oC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLightR 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37oC. Isotype control antibody (blue line) was rabbit IgG1 (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.