Anti TOMM40  

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling TOMM40 with M03166 at 1/25 dilution, followed by DylightR 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing mitochondrion staining on U-2 OS cell line. Cytoplasmic actin is detected with DylightR 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).

All lanes : Anti-TOMM40 Antibody (N-Term) at 1:2000 dilution
Lane 1: Human brain lysate
Lane 2: 293 whole cell lysate
Lane 3: U-87 MG whole cell lysate
Lane 4: Hut78 whole cell lysate
Lane 5: Hela whole cell lysate
Lane 6: A431 whole cell lysate
Lane 7: HepG2 whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 38 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing HeLa cells stained with M03166 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M03166, 1:25 dilution) for 60 min at 37oC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLightR 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37oC. Isotype control antibody (blue line) was rabbit IgG1 (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.