FlexAble CoraLite(R) Plus 647 Antibody Labeling Kit for Mouse IgG2b

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin antibody labeled with FlexAble CoraLite Plus 488 Kit (KFA061, green). Nuclei were stained with DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin antibody labeled with FlexAble CoraLite Plus 488 Kit (KFA061, green), mouse IgG2b anti-PAF49 primary and anti-mouse IgG secondary antibody Alexa Fluor 568 (red) and CoraLite 647-conjugated HDAC2 antibody (CL647-67615, grey). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-HDAC2 (676165-1-Ig) labeled with FlexAble CoraLite Plus 488 Kit (KFA061, green), mouse IgG2b anti-Lamin primary and anti-mouse IgG secondary antibody Alexa Fluor 568 (red) and anti-Tom20 (66777-1-Ig) labeled with FlexAble ？CoraLite Plus 647 Kit (KFA063, magenta). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin antibody labeled with FlexAble CoraLite Plus 488 Kit (KFA061, green), anti-Lamin antibody labeled with FlexAble CoraLite Plus 555 Kit (KFA062, red) and anti-PAF49 antibody labeled with FlexAble CoraLite Plus 647 Kit (KFA063, yellow). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA022) and anti-human CD4 (clone OKT4, 65134-1-Ig) labeled with FlexAble CoraLite Plus 488 Kit (KFA061).？

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 (clone HI30, 65109-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA022) and anti-human CD20 (clone 2G7, 65085-1-Ig) labeled with FlexAble CoraLite Plus 488 Kit (KFA061).？

WB of HeLa and HEK-293 cell lysates: HeLa and HEK-293 Cell lysates were detected with anti-Beta Actin (66009-1-Ig) labeled with FlexAble CoraLite Plus 488 Kit (KFA061, blue); anti-Alpha Tubulin (66031-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA062, red); and anti-ANP32B (66160-1-Ig) labeled with FlexAble CoraLite Plus 750 Kit (KFA064, green).