Anti GSTM3

Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate
All lanes: GSTM3 antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 27 KDa
Observed band size: 27 KDa
Exposure time：10min

IHC image of CSB-MA009982A0m diluted at 1:100 and staining in paraffin-embedded human testes tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Overlay Peak curve showing Hela cells stained with CSB-MA009982A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.