別品名 |
TFI; EPI; LACI; TFPI1; Lipoprotein-Associated Coagulation Inhibitor; Extrinsic pathway inhibitor
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種由来 |
Human
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適用 |
Western Blot Immunohistochemistry Immuno Fluorescence Immunocytochemistry (cell) Flow Cytometry Immunoprecipitation
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免疫動物 |
Escherichia coli
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Briefly, recombinant TFPI antibody were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to recombinant human TFPI-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with HRP labelled secondary antibody wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450/630nm immediately. The binding activity of rh TFPI and recombinant TFPI antibody was shown in Figure 1, and this effect was in a dose dependent manner.
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Briefly, recombinant TFPI antibody were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to recombinant human TFPI-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with HRP labelled secondary antibody wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450/630nm immediately. The binding activity of rh TFPI and recombinant TFPI antibody was shown in Figure 1, and this effect was in a dose dependent manner.
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メーカー |
品番 |
包装 |
WLS
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RAA394HU64
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200 UL
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※表示価格について
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