| 別品名 |
Anti-SARS-CoV-2 Spike P681R (Delta Variant) antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein, Delta variant
|
| 種由来 |
SARS-CoV-2
|
| 標識物 |
Unlabeled
|
| 精製度 |
Affinity Purified
|
| 適用 |
Enzyme Linked Immunosorbent Assay
IHC paraffin embedding section
|
| 免疫動物 |
Mouse
|
| 抗体クラス |
IgG1
|
| クローン |
5H4C5
|
| GENE ID |
43740568
|
| Accession No.(Gene/Protein) |
QHD43416, P0DTC2
|
| Gene Symbol |
S
|
| 形状 |
液状
|
| 参考文献 |
Zhang et al. JAMA 2021, 325 (13): 1324-26.
Reardon. Nature 2021.
Delphine. Nature 2021, 596 (7871): 276-280.
|
|
※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1 Immunohistochemistry Validation of Spike in Delta Variant COVID-19 Patient Lung Tissue
Immunohistochemical analysis of paraffin-embedded patient lung tissue using anti-Spike P681R antibody (PM-9677) at 20 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in the Delta variant COVID-19 patient lung, but not in WT COVID-19 patient lung.
Figure 2 SARS-Cov-2 Spike P681R (Delta Variant) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISA
Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 μg/mL, incubated at 4 ˚C overnight.
Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, PM-9677, 1 μg/mL, incubated at RT for 1 hr.
Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.
Figure 3 ELISA Validation of Delta Variant Spike S1 Antibodies with SARS-CoV-2 Delta Variant Spike S1 Protein
Coating Antigen: SARS-CoV-2 spike S1 proteins, WT, Alpha and Delta variants, 1 μg/mL, incubated at 4 ˚C overnight.
Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, PM-9677, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.
Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.
SARS-CoV-2 Spike P681R (Delta Variant) antibody specifically detects spike S1 protein of Delta variant, but not WT and Alpha variant.
Figure 4 ELISA Validation of Delta Variant Spike S1 Antibodies with Mutant and WT Peptide
Coating Antigen: SARS-CoV-2 spike S1 peptides: WT (9091P), Alpha variant (9359P), and Delta variant (9673P), 1 μg/mL, incubated at 4 ˚C overnight.
Detection Antibodies: SARS-CoV-2 delta spike P681R antibody, PM-9677, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.
Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.
SARS-CoV-2 Spike P681R (Delta Variant) antibody detects Delta variant spike S1 peptide (681R, 9673P), but not WT peptide (681P, 9091P) and Alpha variant spike S1 peptide (681H, 9359P).
|
|
|
|
Figure 1 Immunohistochemistry Validation of Spike in Delta Variant COVID-19 Patient Lung Tissue
Immunohistochemical analysis of paraffin-embedded patient lung tissue using anti-Spike P681R antibody (PM-9677) at 20 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in the Delta variant COVID-19 patient lung, but not in WT COVID-19 patient lung.
|
|
|
| メーカー |
品番 |
包装 |
|
PSC
|
PM-9677
|
0.1 MG
|
※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
3週間程度
|
| 保存温度 |
-２０℃
|
|
