FlexAble CoraLite(R) Plus 647 Antibody Labeling Kit for Mouse IgG1, Trial Size

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite Plus 647 Kit (KFA023) and anti-CD4 (clone RPA-T4, 65143-1-Ig) labeled with FlexAble CoraLite Plus 750 Kit (KFA024).

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite Plus 647 Kit (KFA023) and anti-CD8 (clone RPA-T8, 65144-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA022).

WB of HeLa cell lysates: HeLa cell lysates were detected with anti-GAPDH (10494-1-AP) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, green) and anti-NUDT21 (66335-1-Ig) labeled with FlexAble CoraLite Plus 647 Kit (KFA023, red).

WB of A431 cell lysate: siRNA transfected A431 cell lysates were detected with anti-P53 (80077-1-RR) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, green) and anti-PCNA (60097-1-Ig) labeled with FlexAble CoraLite Plus 647 Kit (KFA023, red).

Immunofluorescence of human kidney: FFPE human kidney sections were stained with anti-Calbindin (14479-1-AP) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, yellow), anti-ACE2 (66699-1-Ig) labeled with FlexAble CoraLite Plus 647 Kit (KFA023, magenta), CoraLite488-conjugated Podocalyxin antibody (CL488-18150, green) and DAPI (blue).

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-HSP60 (66041-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA022, yellow), anti-GORASP2 (66627-1-Ig) labeled with FlexAble CoraLite Plus 647 Kit (KFA023, cyan) and DAPI (blue). ？Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Vimentin labeled with FlexAble CoraLite Plus 555 Kit (KFA022, yellow) and anti-hnRNP labeled with FlexAble CoraLite Plus 647 Kit (KFA023, cyan).？ Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.