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Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 labeled with FlexAble CoraLite Plus 647 Kit (KFA003) and anti-CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA022).
Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 labeled with FlexAble CoraLite Plus 647 Kit (KFA003) and anti-CD4 (clone RPA-T4, 65143-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA022).
WB of HEK293 cell lysates: siRNA transfected HEK293 cell lysates were detected with anti-GNA13 (67188-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA022,green) and anti-PCNA (60097-1-Ig) labeled with FlexAble CoraLite Plus 647 Kit (KFA023, red).
WB of HepG2 cell lysates: siRNA transfected HepG2 cell lysates were detected with anti-CCDC111 (29824-1-AP) labeled with FlexAble CoraLite Plus 647 Kit (KFA003, red) and anti-PCNA (60097-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA022, green).
Immunofluorescence of rat brain tissue: FFPE rat brain tissue sections were stained with anti-NeuN (66836-1-Ig) labeled with FlexAble CoraLite Plus 647 Kit (KFA023, magenta), anti-TUBB3 (66375-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA022, orange), and CoraLite488-conjugated GFAP antibody (CL488-60190, green).
Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Lamin B1 (66095-1-Ig) labeled with FlexAble CoraLite 488 Kit (KFA021, green), anti-HSP60 (66041-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA022, red) and anti-GORASP2 (66627-1-Ig) labeled with FlexAble CoraLite Plus 647 Kit (KFA023, cyan). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
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