FlexAble CoraLite^(R) Plus 488 Antibody Labeling Kit for Mouse IgG1  

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with 0.5 μg anti-human CD3 antibody (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite 488 Kit (KFA021).

WB of HEK293 cell lysates: HEK293 cell lysates were detected with anti-GAPDH (10494-1-AP) labeled with FlexAble CoraLite Plus 750 Kit (KFA004, red) and anti-NUDT21 (66335-1-Ig) labeled with FlexAble CoraLite 488 Kit (KFA021, green).

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Ki67 labeled with FlexAble CoraLite 488 Kit (KFA021, green) and anti-Tubulin alpha labeled with FlexAble CoraLite Plus 647 Kit (KFA023, magenta). Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Lamin B1 (66095-1-Ig) labeled with FlexAble CoraLite 488 Kit (KFA021, green) and anti-HSP60 (66041-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA022, blue), anti-GORASP2 (66627-1-Ig) labeled with FlexAble CoraLite Plus 647 Kit (KFA023, yellow) and anti-Tubulin alpha labeled with FlexAble CoraLite Plus 750 Kit (KFA024, grey). Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Lamin B1 (66095-1-Ig) labeled with FlexAble CoraLite 488 Kit (KFA021, green), anti-HSP60 (66041-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA022, red) and anti-GORASP2 (66627-1-Ig) labeled with FlexAble CoraLite Plus 647 Kit (KFA023, cyan). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin alpha labeled with FlexAble CoraLite 488 Kit (KFA021, green) and DAPI (blue). Epifluorescence images were acquired with a 20x objective and post-processed.