FlexAble CoraLite(R) Plus 647 Antibody Labeling Kit for Rabbit IgG, Trial Size

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 labeled with FlexAble CoraLite Plus 647 Kit (KFA003) and anti-CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA022).

Flow cytometry of Jurkat cells. 1X10^6 Jurkat cells were stained with 0.5 μg anti-HSP90 antibody (13171-1-AP) labeled with FlexAble CoraLite Plus 647 Kit (KFA003, cyan) or with isotype control antibody labeled with FlexAble CoraLite Plus 647 Kit (KFA003, red).

WB of HeLa cell lysates: HeLa cell lysates were detected with anti-GAPDH (10494-1-AP) labeled with FlexAble CoraLite Plus 647 Kit (KFA003, red) and anti-NUDT21 (66335-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA022, green).

WB of HepG2 cell lysates: siRNA transfected HepG2 cell lysates were detected with anti-CCDC111 (29824-1-AP) labeled with FlexAble CoraLite Plus 647 Kit (KFA003, red) and anti-PCNA (60097-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA022, green).

Immunofluorescence of rat brain tissue: FFPE rat brain tissue sections were stained with anti-MAP2 (17490-1-AP) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, orange), anti-GFAP (16825-1-AP) labeled with FlexAble CoraLite Plus 647 Kit (KFA003, magenta) and CoraLite488-conjugated TDP-43 antibody (CL488-10782, green).

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-COXIV (11242-1-AP) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, yellow), anti-GM130 (11308-1-AP) labeled with FlexAble CoraLite Plus 647 Kit (KFA003, magenta) and DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TDP-43 (10782-2-AP) labeled with FlexAble CoraLite 488 Kit (KFA001, green), anti-Tom20 (11802-1-AP) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, red) and anti-Lamin B1 (12987-1-AP) labeled with FlexAble CoraLite Plus 647 Kit (KFA003, cyan). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.