FlexAble CoraLite(R) Plus 555 Antibody Labeling Kit for Rabbit IgG

Flow cytometry of Jurkat cells. 1X10^6 Jurkat cells were stained with 0.5 μg anti-HSP90 antibody (13171-1-AP) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, cyan) or with isotype control antibody labeled with FlexAble CoraLite Plus 555 Kit (KFA002, red).

WB of HeLa cell lysates: HeLa cell lysates were detected with anti-GAPDH (10494-1-AP) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, green) and anti-NUDT21 (66335-1-Ig) labeled with FlexAble CoraLite Plus 647 Kit (KFA023, red).

WB of A431 cell lysate: siRNA transfected A431 cell lysates were detected with anti-P53 (80077-1-RR) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, green) and anti-PCNA (60097-1-Ig) labeled with FlexAble CoraLite Plus 647 Kit (KFA023, red).

Immunofluorescence of human kidney: FFPE human kidney sections were stained with anti-Calbindin (14479-1-AP) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, yellow), anti-ACE2 (66699-1-Ig) labeled with FlexAble CoraLite Plus 647 Kit (KFA023, magenta), CoraLite488-conjugated Podocalyxin antibody (CL488-18150, green) and DAPI (blue).

Immunofluorescence of rat brain tissue: FFPE rat brain tissue sections were stained with anti-MAP2 (17490-1-AP) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, orange), anti-GFAP (16825-1-AP) labeled with FlexAble CoraLite Plus 647 Kit (KFA003, magenta) and CoraLite488-conjugated TDP-43 antibody (CL488-10782, green).

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TOM20 (11802-1-AP) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, yellow) and anti-Lamin B1 (12987-1-AP) labeled with FlexAble CoraLite Plus 647 Kit (KFA003, cyan). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.