FlexAble CoraLite(R) Plus 488 Antibody Labeling Kit for Rabbit IgG

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with 0.5 μg anti-HSP90 antibody (13171-1-AP) labeled with FlexAble CoraLite 488 Kit (KFA001, cyan) or with isotype control antibody labeled with FlexAble CoraLite 488 Kit (KFA001, red).

WB of HEK293 cell lysates: HEK293 cell lysates were detected with anti-GAPDH (10494-1-AP) labeled with FlexAble CoraLite 488 Kit (KFA001, green) and anti-NUDT21 (66335-1-Ig) labeled with FlexAble CoraLite Plus 750 Kit (KFA024, red).

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tom70 (14528-1-AP) labeled with FlexAble CoraLite 488 Kit (KFA001, green) and anti-Lamin B1 (12987-1-AP) labeled with FlexAble CoraLite Plus 647 Kit (KFA003, cyan). Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TDP43 (10782-2-AP) labeled with FlexAble CoraLite 488 Kit (KFA001, green), anti-TOM20 (11802-1-AP) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, red), anti-Lamin B1 (12987-1-AP) labeled with FlexAble CoraLite Plus 647 Kit (KFA003, magenta) and anti-CD147 labeled with FlexAble CoraLite Plus 750 Kit (KFA004, cyan). Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TDP-43 (10782-2-AP) labeled with FlexAble CoraLite 488 Kit (KFA001, green), anti-Tom20 (11802-1-AP) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, red) and anti-Lamin B1 (12987-1-AP) labeled with FlexAble CoraLite Plus 647 Kit (KFA003, cyan). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TOM70 (14528-1-AP) labeled with FlexAble CoraLite 488 Kit (KFA001, green) and DAPI (blue). Epifluorescence images were acquired with a 20x objective and post-processed.