Anti TSG101

Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate
All lanes: TSG101 antibody at 1:500
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 44, 32 KDa
Observed band size: 44 KDa
Exposure time：5min

Immunofluorescence staining of U251 cells with CSB-MA860355A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Overlay Peak curve showing U251 cells stained with CSB-MA860355A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106 cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.