IL-21

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Recombinant human IL-21 (Cat no: HZ-1319) induces dose-dependent release of IFN-gamma in the NK-92 human cell line. NK-92 cells were treated with increasing concentration of recombinant IL-21 for 72 hours before supernatant collection. The supernatant was tested for IFN gamma using Proteintech’s AuthentiKine Human IFN-gamma ELISA Kit (KE00146). The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range in 0.2-1 ng/mL.