Anti PI 3 Kinase p85 α, phospho Tyr607  

Immunohistochemistry of Rabbit Anti-PI 3 Kinase p85 alpha pY607 Antibody. Tissue: Human Brain Cortex. Fixative: none. Antigen Retrieval: HIER using Citrate Buffer for 20 minutes. Primary Antibody: Anti-PI3 Kinase p85 alpha pY607 at 1:100 for 30mins at RT. Secondary Antibody: Goat Anti-Rabbit Poly IgG HRP Ready-to-Use for 8 mins at RT. Counterstain: Hematoxylin. Substrate: DAB. Results: shows intense intracytoplasmic and intranuclear staining in neurons, glial cells in human brain cortex at both dilutions. There is diffuse mild neuropil staining.

Dot Blot of Rabbit Anti-PI 3 Kinase p85 alpha pY607 Antibody. Dilution Columns: 1-100ng, 2-33.33ng, 3-11.11ng, 4-3.70ng, 5-1.23ng. Row A: PI 3 Kinase p85 alpha pY607-BSA conjugated Peptide. Row B: PI 3 Kinase p85 alpha non-phospho Y607-BSA conjugated Peptide. Row C: STAT3 pY705-BSA conjugated Peptide. Row D: STAT3 non-phospho Y705-BSA conjugated Peptide. Row E: Scrambled Sprouty-BSA conjugated peptide. Row F: BSA only. Primary Antibody: Anti-PI 3 Kinase p85 alpha pY607 at 1μg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) 1:70,000 for 1hr at RT. Blocking: Universal BlockOut Buffer (p/n MB-073). Exposure: 10 sec.