RScript Reverse Transcriptase  

Figure 1. Stability of RScript reverse transcriptase:
The stability RT-qPCR data shows the performance of RScript reverse transcriptase is maintained without any significant alteration in performance at 37 °C after a period of 3 weeks (21 days), demonstrating its high resistance to temperature and time. The percentage of RScript reverse transcriptase activity was calculated by dividing values at each reaction temperature.

A: Bio-Helix RScript reverse transcriptase #RT001-0050
B: Promega Improm II #A3802
C: Thermo Superscript III #18080093
D: Superscript IV #18090010

Figure 2. Enhancement of the thermostability: Reverse transcription of a 1 kb fragment ARHGAP29 RNA using RScript reverse transcriptase or competitor reverse transcriptases were used to carry this experiment. Regardless of the variations in temperatures ( 42 -60 °C) RScript reverse transcriptase shows a stable and superior performance at higher temperatures of up to 60 ℃ compared to other brands. The molecular weight marker used was DNA Ladder 1kb #SDL-1000R.

Red: Bio-HeliX RScript reverse transcriptase #RT001-0050
Green: Improm-II #A3802
Blue: Superscript IV #18090010

Figure 3. Performing RT-qPCR with RScript reverse transcriptase :
At a temperature of 55 °C, RScript reverse transcriptase exceeds performance demonstrating better efficiency and higher cDNA yields. RScript reverse transcriptase shows a stable and superior performance at 55 ℃ compared to other brands. Therefore RScript reverse transcriptase does not encounter significant higher Ct values compared to Improm II and SuperScript IV due to their low amounts of input cDNA.