Anti SOD1, recombinant antibody  

Western Blot
Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, U87 whole cell lysate
All lanes: SOD1 antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 16 kDa
Observed band size: 18 kDa

Overlay histogram showing Jurkat cells stained with CSB-RA829583A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1μg/1*10⁶ cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1μg/1*10⁶ cells) used under the same conditions. Acquisition of >10,000 events was performed.