Anti CREBBP

ELISA results of purified polyclonal Rabbit Anti CREBBP Antibody tested against BSA conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5μg/ml and the X axis represents the Log10 of a 3 fold dilution. This titration is a 4 parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Coating buffer, Goat Anti Rabbit HRP conjugated (p/n 611 103 122) and substrate (p/n TMBE 1000).

Western Blot of Rabbit Anti-CREBBP Antibody. Lane 1: Opal Pre-Stained Marker (p/n MB-210-0500). Lane 2: Mouse Brain Whole Tissue Lysate (p/n W10-000-T004) [+]. Lane 3: Human Eye Tissue Lysate [-]. Load: 35μg lysate/lane on a 7.5% gel. Primary Antibody: Anti-CREBBP at 5μg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:70,000 at RT. Block: Universal Buffer BlockOut (p/n MB-073). Predicted MW: ~265, 494kDa (Mouse), ~265kDa (Human). Observed MW: ~260, 270kDa. Exposure: 60sec.

Western Blot of Rabbit Anti-CREBBP Antibody. Lane 1: Opal Pre-Stained Marker (p/n MB-210-0500). Lane 2: MOLT-4 Whole Cell Lysate (p/n W09-001-GK2). Lane 3: HEK293T Whole Cell Lysate (p/n W09-001-GX5). Lane 4: A549 Whole Cell Lysate (p/n W09-001-372). Lane 5: PC-3 Whole Cell Lysate (p/n W09-001-GV6). Lane 6: Mouse Brain Whole Tissue Lysate (p/n W10-000-T004) [+]. Load: 35ug lysate/lane on a 7.5% gel. Primary Antibody: Anti-CREBBP at 5ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:70,000 at RT. Block: Universal Buffer BlockOut (p/n MB-073). Predicted MW: ~265, 494kDa (Mouse), ~265kDa (Human). Observed MW: >245kDa. Exposure: 30sec.