Anti CREBBP  

Western Blot of Rabbit Anti-CREBBP Antibody. Lane 1: Opal Pre-Stained Marker (p/n MB-210-0500). Lane 2: Mouse Brain Whole Tissue Lysate (p/n W10-000-T004) [+]. Lane 3: Human Eye Tissue Lysate [-]. Load: 35μg lysate/lane on a 7.5% gel. Primary Antibody: Anti-CREBBP at 5μg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:70,000 at RT. Block: Universal Buffer BlockOut (p/n MB-073). Predicted MW: ~265, 494kDa (Mouse), ~265kDa (Human). Observed MW: ~260, 270kDa. Exposure: 60sec.

Western Blot of Rabbit Anti-CREBBP Antibody. Lane 1: Opal Pre-Stained Marker (p/n MB-210-0500). Lane 2: MOLT-4 Whole Cell Lysate (p/n W09-001-GK2). Lane 3: HEK293T Whole Cell Lysate (p/n W09-001-GX5). Lane 4: A549 Whole Cell Lysate (p/n W09-001-372). Lane 5: PC-3 Whole Cell Lysate (p/n W09-001-GV6). Lane 6: Mouse Brain Whole Tissue Lysate (p/n W10-000-T004) [+]. Load: 35μg lysate/lane on a 7.5% gel. Primary Antibody: Anti-CREBBP at 5μg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:70,000 at RT. Block: Universal Buffer BlockOut (p/n MB-073). Predicted MW: ~265, 494kDa (Mouse), ~265kDa (Human). Observed MW: >245kDa. Exposure: 30sec.

ELISA results of purified polyclonal Rabbit Anti-CREBBP Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1μg of conjugate. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Coating buffer, Goat Anti-Rabbit HRP conjugated (p/n 611-103-122) and substrate (p/n TMBE-1000).