別品名 |
Goant Anti-Human Chorionic Gonadotropin Alpha Antibody, Goat anti-hCGa Antibody, α-hCG, a-hCG, alpha-hCG, Glycoprotein hormones alpha chain, Anterior pituitary glycoprotein hormones common subunit alpha, Choriogonadotropin alpha chain, Chorionic gonadotrophin subunit alpha, CG-alpha, Follicle-stimulating hormone alpha chain, FSH-alpha, Follitropin alpha chain, Luteinizing hormone alpha chain, LSH-alpha, Lutropin alpha chain, Thyroid-stimulating hormone alpha chain, TSH-alpha, Thyrotropin alpha chain
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay
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免疫動物 |
Goat
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抗体クラス |
IgG
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交差種 |
Human
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GENE ID |
1081
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Accession No.(Gene/Protein) |
NP_000726, P01215
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形状 |
滅菌済み液状品
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
ELISA results of Goat Anti Human Chorionic Gonadotropin Alpha Chain Antibody reactive against alpha hCG and full hCG. Each well was coated in duplicate at 10ug/mL of alpha hCG (RED) and full hCG (BLUE) protein. The starting dilution of antibody was 5μg/mL and the X axis represents the Log10 of a 3 fold dilution. This titration is a 4 parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking Buffer (MB 066 1000), Donkey Anti Goat IgG MX8 HRP Conjugated Secondary (605 703 125 0025), and TMBE 1000 substrate.
Western Blot of Goat Anti-Human Chorionic Gonadotropin Alpha Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: alpha hCG (0.05ug) [+]. Lane 3: alpha hCG/U-20S WCL (p/n W09-001-374) (0.05ug/20ug) [+]. Lane 4: U-20S WCL (p/n W09-001-374) (20ug) [-]. Lane 5: HeLa WCL (p/n W09-000-364) (35ug) [+]. Primary Antibody: Anti-α-hCG at 1:1000 overnight at 2-8C. Secondary Antibody: Donkey Anti-Goat IgG mx8 HRP Conjugated (p/n 605-703-125-0025) at 1:40,000 for 30 mins at RT. Block: BlockOut Buffer (p/n MB-073). Exposure: 30 sec. Predicted MW: alpha subunit ~14.9kDa, full hCG ~37kDa, luteinizing hormone (LH) ~29kDa, thyroid stimulating hormone (TSH) ~28kDa, follicle stimulating hormone (FSH) ~30kDa. Observed MW: alpha subunit ~15-20kDa, full hCG ~35kDa.
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ELISA results of Goat Anti Human Chorionic Gonadotropin Alpha Chain Antibody reactive against alpha hCG and full hCG. Each well was coated in duplicate at 10ug/mL of alpha hCG (RED) and full hCG (BLUE) protein. The starting dilution of antibody was 5μg/mL and the X axis represents the Log10 of a 3 fold dilution. This titration is a 4 parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking Buffer (MB 066 1000), Donkey Anti Goat IgG MX8 HRP Conjugated Secondary (605 703 125 0025), and TMBE 1000 substrate.
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メーカー |
品番 |
包装 |
RKL
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600-101-MR6S
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25 UL
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
-20℃
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