別品名 |
Fibroblast growth factor 7; FGF-7; Heparin-binding growth factor 7; HBGF-7; Keratinocyte growth factor; KGF; Fgf7; Fgf-7, Kgf;
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抗原部位 |
a.a.54-178
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay
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免疫動物 |
Rabbit
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抗体クラス |
IgG
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交差種 |
Human
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GENE ID |
2252
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Accession No.(Gene/Protein) |
P21781
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Gene Symbol |
FGF7
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分子量 |
22 kDa
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形状 |
凍結乾燥品
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参考文献 |
1. Kelley MJ, Pech M, Seuanez HN, Rubin JS, O'Brien SJ, Aaronson SA (Nov 1992). "Emergence of the keratinocyte growth factor multigene family during the great ape radiation". Proc Natl Acad Sci U S A 89 (19): 9287-91. 2. Mattei MG, deLapeyriere O, Bresnick J, Dickson C, Birnbaum D, Mason I (Jun 1995). "Mouse Fgf7 (fibroblast growth factor 7) and Fgf8 (fibroblast growth factor 8) genes map to chromosomes 2 and 19 respectively". Mamm Genome 6 (3): 196-7.
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※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1. Western blot analysis of KGF/FGF7 using anti-KGF/FGF7 antibody (A01931-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KGF/FGF7 antigen affinity purified polyclonal antibody (Catalog # A01931-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KGF/FGF7 at approximately 22KD. The expected band size for KGF/FGF7 is at 22KD.
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Figure 1. Western blot analysis of KGF/FGF7 using anti-KGF/FGF7 antibody (A01931-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KGF/FGF7 antigen affinity purified polyclonal antibody (Catalog # A01931-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KGF/FGF7 at approximately 22KD. The expected band size for KGF/FGF7 is at 22KD.
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メーカー |
品番 |
包装 |
BBT
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A01931-1
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100 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
1週間程度
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保存温度 |
-20℃
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