| 適用 |
Enzyme Linked Immunosorbent Assay
Dot Blot
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| 免疫動物 |
Llama
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| クローン |
RFP40
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| Accession No.(Gene/Protein) |
Q9U6Y8
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| 発現系 |
大腸菌
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western Blot Results of Anti RFP (VHH) Single Domain Antibody. Lane 1: Opal Prestained Molecular Weight (p/n MB 210 0500). Lane 2: HEK293 Whole Cell Lysate (p/n W09 000 365) [10ug] ( ). Lane 3: HEK293 Whole Cell Lysate (p/n W09 000 365) [10ug] + GFP rec. protein (p/n 000 001 215) [1ug] ( ). Lane 4: HEK293 Whole Cell Lysate (p/n W09 000 365) [10ug] + RFP rec. protein (p/n 000 001 379) [1ug] (+). Lane 5: RFP rec. protein (p/n 000 001 379) [1ug] (+).Primary Antibody: Anti RFP (VHH) Single Domain Antibody (p/n 400 001 MS3) at 1:1000 for overnight at 4C. Secondary Antibody: Mouse Anti 6x His DL649 conjugated (p/n 200 343 382) at 1:10,000 for 1hr at RT. Block: Blocking Buffer for Fluorescent Western Blotting (p/n MB 070) overnight at 4C. Expected MW: ~27kDa for RFP protein.
ELISA Results of purified recombinant Anti-RFP Single Domain Antibody reactive against RFP. The working dilution for clone RFP40 is 1:31,000. Each well was coated in duplicate with 1.0μg of RFP (p/n 000-001-379). The starting dilution of antibody was 45μg/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS blocking buffer (p/n MB-066), Mouse Anti-FLAG Tag HRP conjugated Antibody (p/n 200-303-383) and TMB substrate (p/n TMBE-1000).
Dot Blot of Anti-RFP Single Domain Antibody. Row A: Recombinant RFP protein (p/n 000-001-379). Amount Dotted: (1) 200, (2) 100, (3) 50, (4) 25, (5) 12.5ng. Primary Antibody: Anti-RFP (VhH) 1.0ug/mL for 1.0hr at RT. Secondary Antibody: Mouse Anti-Flag Peroxidase (p/n 200-303-383) at 1:5000 for 30 mins at RT. Block: BlockOut Buffer (p/n MB-073). Exposure: 10 seconds.
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Western Blot Results of Anti RFP (VHH) Single Domain Antibody. Lane 1: Opal Prestained Molecular Weight (p/n MB 210 0500). Lane 2: HEK293 Whole Cell Lysate (p/n W09 000 365) [10ug] ( ). Lane 3: HEK293 Whole Cell Lysate (p/n W09 000 365) [10ug] + GFP rec. protein (p/n 000 001 215) [1ug] ( ). Lane 4: HEK293 Whole Cell Lysate (p/n W09 000 365) [10ug] + RFP rec. protein (p/n 000 001 379) [1ug] (+). Lane 5: RFP rec. protein (p/n 000 001 379) [1ug] (+).Primary Antibody: Anti RFP (VHH) Single Domain Antibody (p/n 400 001 MS3) at 1:1000 for overnight at 4C. Secondary Antibody: Mouse Anti 6x His DL649 conjugated (p/n 200 343 382) at 1:10,000 for 1hr at RT. Block: Blocking Buffer for Fluorescent Western Blotting (p/n MB 070) overnight at 4C. Expected MW: ~27kDa for RFP protein.
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| メーカー |
品番 |
包装 |
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RKL
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400-001-MS3
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50 UG
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※表示価格について
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