|
※サムネイル画像をクリックすると拡大画像が表示されます。
Western Blot Results of Anti-RFP (VHH) Single Domain Antibody. Lane 1: Opal Prestained Molecular Weight (p/n MB-210-0500). Lane 2: HEK293 Whole Cell Lysate (p/n W09-000-365) [10ug] (-). Lane 3: HEK293 Whole Cell Lysate (p/n W09-000-365) [10ug] + GFP rec. protein (p/n 000-001-215) [1ug] (-). Lane 4: HEK293 Whole Cell Lysate (p/n W09-000-365) [10ug] + RFP rec. protein (p/n 000-001-379) [1ug] (+). Lane 5: RFP rec. protein (p/n 000-001-379) [1ug] (+).Primary Antibody: Anti-RFP (VHH) Single Domain Antibody (p/n 400-001-MS3) at 1:1000 for overnight at 4°C. Secondary Antibody: Mouse Anti-6x His DL649 conjugated (p/n 200-343-382) at 1:10,000 for 1hr at RT. Block: Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) overnight at 4°C. Expected MW: ~27kDa for RFP protein.
ELISA Results of purified recombinant Anti-RFP Single Domain Antibody reactive against RFP. The working dilution for clone RFP40 is 1:31,000. Each well was coated in duplicate with 1.0μg of RFP (p/n 000-001-379). The starting dilution of antibody was 45μg/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS blocking buffer (p/n MB-066), Mouse Anti-FLAG Tag HRP conjugated Antibody (p/n 200-303-383) and TMB substrate (p/n TMBE-1000).
Dot Blot of Anti-RFP Single Domain Antibody. Row A: Recombinant RFP protein (p/n 000-001-379). Amount Dotted: (1) 200, (2) 100, (3) 50, (4) 25, (5) 12.5ng. Primary Antibody: Anti-RFP (VhH) 1.0μg/mL for 1.0hr at RT. Secondary Antibody: Mouse Anti-Flag Peroxidase (p/n 200-303-383) at 1:5000 for 30 mins at RT. Block: BlockOut Buffer (p/n MB-073). Exposure: 10 seconds.
|
|
|
|
Western Blot Results of Anti-RFP (VHH) Single Domain Antibody. Lane 1: Opal Prestained Molecular Weight (p/n MB-210-0500). Lane 2: HEK293 Whole Cell Lysate (p/n W09-000-365) [10ug] (-). Lane 3: HEK293 Whole Cell Lysate (p/n W09-000-365) [10ug] + GFP rec. protein (p/n 000-001-215) [1ug] (-). Lane 4: HEK293 Whole Cell Lysate (p/n W09-000-365) [10ug] + RFP rec. protein (p/n 000-001-379) [1ug] (+). Lane 5: RFP rec. protein (p/n 000-001-379) [1ug] (+).Primary Antibody: Anti-RFP (VHH) Single Domain Antibody (p/n 400-001-MS3) at 1:1000 for overnight at 4°C. Secondary Antibody: Mouse Anti-6x His DL649 conjugated (p/n 200-343-382) at 1:10,000 for 1hr at RT. Block: Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) overnight at 4°C. Expected MW: ~27kDa for RFP protein.
|
|
| 別品名 |
recombinant anti-RFP antibody, Red Fluorescent Protein antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
|
| 種由来 |
Escherichia coli
|
| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Dot Blot
|
| クローン |
RFP40
|
| 標識物 |
Unlabeled
|
| Accession No.(Gene/Protein) |
Q9U6Y8
|
| Gene Symbol |
DsRed
|
| 発現系 |
大腸菌
|
|
| メーカー |
品番 |
包装 |
|
RKL
|
400-001-MS3
|
50 UG
|
※表示価格について
|
※当社では商品情報の適切な管理に努めておりますが、表示される法規制情報は最新でない可能性があります。
また法規制情報の表示が無いものは、必ずしも法規制に非該当であることを示すものではありません。
商品のお届け前に最新の製品法規制情報をお求めの際はこちらへお問い合わせください。
|
※当社取り扱いの試薬・機器製品および受託サービス・創薬支援サービス（納品物、解析データ等）は、研究用としてのみ販売しております。
人や動物の医療用・臨床診断用・食品用としては、使用しないように、十分ご注意ください。
法規制欄に体外診断用医薬品と記載のものは除きます。
|
|
※リンク先での文献等のダウンロードに際しましては、掲載元の規約遵守をお願いします。
|
