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Figure 1. Western blot analysis of Alpha B Crystallin/CRYAB using anti-Alpha B Crystallin/CRYAB antibody (A03496-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat eye tissue lysates,
Lane 2: mouse eye tissue lysates,
Lane 3: rat heart tissue lysates,
Lane 4: mouse heart tissue lysates,
Lane 5: monkey heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha B Crystallin/CRYAB antigen affinity purified polyclonal antibody (Catalog # A03496-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002)？with Tanon 5200 system. A specific band was detected for Alpha B Crystallin/CRYAB2 at approximately 20KD. The expected band size for Alpha B Crystallin/CRYAB is at 20KD.
Figure 10. IHC analysis of Alpha B Crystallin/CRYAB using anti-Alpha B Crystallin/CRYAB antibody (A03496-2).
Alpha B Crystallin/CRYAB was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Alpha B Crystallin/CRYAB Antibody (A03496-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 2. IHC analysis of Alpha B Crystallin/CRYAB using anti-Alpha B Crystallin/CRYAB antibody (A03496-2).
Alpha B Crystallin/CRYAB was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Alpha B Crystallin/CRYAB Antibody (A03496-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of Alpha B Crystallin/CRYAB using anti-Alpha B Crystallin/CRYAB antibody (A03496-2).
Alpha B Crystallin/CRYAB was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Alpha B Crystallin/CRYAB Antibody (A03496-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis of Alpha B Crystallin/CRYAB using anti-Alpha B Crystallin/CRYAB antibody (A03496-2).
Alpha B Crystallin/CRYAB was detected in paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Alpha B Crystallin/CRYAB Antibody (A03496-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 5. IHC analysis of Alpha B Crystallin/CRYAB using anti-Alpha B Crystallin/CRYAB antibody (A03496-2).
Alpha B Crystallin/CRYAB was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Alpha B Crystallin/CRYAB Antibody (A03496-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 6. IHC analysis of Alpha B Crystallin/CRYAB using anti-Alpha B Crystallin/CRYAB antibody (A03496-2).
Alpha B Crystallin/CRYAB was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Alpha B Crystallin/CRYAB Antibody (A03496-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 7. IHC analysis of Alpha B Crystallin/CRYAB using anti-Alpha B Crystallin/CRYAB antibody (A03496-2).
Alpha B Crystallin/CRYAB was detected in paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Alpha B Crystallin/CRYAB Antibody (A03496-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 8. IHC analysis of Alpha B Crystallin/CRYAB using anti-Alpha B Crystallin/CRYAB antibody (A03496-2).
Alpha B Crystallin/CRYAB was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Alpha B Crystallin/CRYAB Antibody (A03496-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 9. IHC analysis of Alpha B Crystallin/CRYAB using anti-Alpha B Crystallin/CRYAB antibody (A03496-2).
Alpha B Crystallin/CRYAB was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Alpha B Crystallin/CRYAB Antibody (A03496-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 1. Western blot analysis of Alpha B Crystallin/CRYAB using anti-Alpha B Crystallin/CRYAB antibody (A03496-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat eye tissue lysates,
Lane 2: mouse eye tissue lysates,
Lane 3: rat heart tissue lysates,
Lane 4: mouse heart tissue lysates,
Lane 5: monkey heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha B Crystallin/CRYAB antigen affinity purified polyclonal antibody (Catalog # A03496-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002)？with Tanon 5200 system. A specific band was detected for Alpha B Crystallin/CRYAB2 at approximately 20KD. The expected band size for Alpha B Crystallin/CRYAB is at 20KD.
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| 別品名 |
Calpain-2 catalytic subunit;3.4.22.53;Calcium-activated neutral proteinase 2;CANP 2;Calpain M-type;Calpain large polypeptide L2;Calpain-2 large subunit;Millimolar-calpain;M-calpain;CAPN2;CANPL2;
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| 種由来 |
Human
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| 交差種 |
Human
Mouse
Rat
Monkey
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immunohistochemistry
Immuno Fluorescence
Immunocytochemistry (cell)
Flow Cytometry
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| 免疫動物 |
Rabbit
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| 抗体クラス |
IgG
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| 抗原部位 |
a.a.30-151
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| GENE ID |
1410
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| Accession No.(Gene/Protein) |
P02511
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| Gene Symbol |
CRYAB
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| 分子量 |
79995 MW
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| 概要 |
Boster Bio Anti-Alpha B Crystallin/CRYAB Antibody Picoband® catalog # A03496-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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| 参考文献 |
1. Berry, V., Francis, P., Reddy, M. A., Collyer, D., Vithana, E., MacKay, I., Dawson, G., Carey, A. H., Moore, A., Bhattacharya, S. S., Quinlan, R. A. Alpha-B crystallin gene (CRYAB) mutation causes dominant congenital posterior polar cataract in humans. Am. J. Hum. Genet. 69: 1141-1145, 2001.
2. Brakenhoff, R. H., Geurts van Kessel, A. H. M., Oldenburg, M., Wijnen, J. T., Bloemendal, H., Meera Khan, P., Schoenmakers, J. G. G. Human alpha-B-crystallin (CRYA2) gene mapped to chromosome 11q12-q23. Hum. Genet. 85: 237-240, 1990.
3. Chavez Zobel, A. T., Loranger, A., Marceau, N., Theriault, J. R., Lambert, H., Landry, J. Distinct chaperone mechanisms can delay the formation of aggresomes by the myopathy-causing R120G alpha-B-crystallin mutant. Hum. Molec. Genet. 12: 1609-1620, 2003.
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| メーカー |
品番 |
包装 |
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BBT
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A03496-2
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 法規制 |
毒
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| 保存温度 |
-２０℃
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