標識物 |
Unlabeled
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精製度 |
Ig fraction - Protein A
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay Immuno Fluorescence Flow Cytometry Immunoprecipitation
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免疫動物 |
Mouse
|
抗体クラス |
IgG2b
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クローン |
18B11H6
|
交差種 |
All Species
|
形状 |
液状
|
※サムネイル画像をクリックすると拡大画像が表示されます。
Western Blot Positive WB detected in: 3 different overexpression lysates with HA tagged All lanes: HA-Tag antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 35, 35, 48 kDa Observed band size: 35, 35, 48 kDa
Western Blot Positive WB detected in: HA-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng All lanes: HA-Tag antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 35 kDa Observed band size: 35 kDa
Western Blot Positive WB detected in: HA-tagged fusion protein All lanes: HA-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 35 kDa Observed band size: 35 kDa
Western Blot Positive WB detected in: 6 different recombinant proteins with HA tagged, Hela whole cell lysate, 3T3 whole cell lysate All lanes: HA-Tag antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution
Immunofluorescence staining of 293F cells with CSB-MA000141M0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescence staining of 293F transfected cells with CSB-MA000141M0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescence staining of 293F cells and 293F transfected cells with Company A, Company B, Company C, Company D, Company E, CSB-MA000141M0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunoprecipitating HA-Tag in 293F transfected whole cell lysateLane 1: Mouse control IgG (1μg) instead of CSB-MA000141M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-MA009476A0m (5μg) + 293F transfected whole cell lysate (500μg)Lane 3: 293F transfected whole cell lysate (20μg)
Immunoprecipitating HA-Tag in 293F transfected whole cell lysateLane 1: Mouse control IgG (1μg) instead of CSB-MA000141M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: Company A (5μg), Company B (5μg),Company C (5μg),Company D (5μg),Company E (5μg),CSB-MA009476A0m (5μg) + 293F transfected whole cell lysate (500μg)Lane 3: 293F transfected whole cell lysate (20μg)
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Western Blot Positive WB detected in: 3 different overexpression lysates with HA tagged All lanes: HA-Tag antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 35, 35, 48 kDa Observed band size: 35, 35, 48 kDa
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メーカー |
品番 |
包装 |
CSB
|
CSB-MA000141M0M
|
100 UL
|
※表示価格について
当社在庫 |
なし
|
納期目安 |
2週間程度
|
保存温度 |
-20℃
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