別品名 |
REPA2, RPA32, RPA34, Replication protein A 32 kDa subunit, Replication factor A protein 2, RP-A p32
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抗原部位 |
a.a.80-270
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot Immunohistochemistry Immuno Fluorescence Immunoprecipitation
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免疫動物 |
Rabbit
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抗体クラス |
IgG
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交差種 |
Human Mouse Rat
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GENE ID |
6118
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Gene Symbol |
RPA2
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分子量 |
Observed: 32kDa
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形状 |
液状
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その他 |
[Swiss-Prot No]P15927%%[ポジティブコントロール]293T, 293T+UV, NIH/3T3, C6
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Western blot analysis of extracts of various cell lines, using RPA2 antibody (18-562) at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human kidney cancer using RPA2 Antibody (18-562) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using RPA2 antibody (18-562). Green:GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 1ug RPA2 antibody (18-562). Western blot was performed from the immunoprecipitate using RPA2 antibody (18-562) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using RPA2 antibody (18-562) at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
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メーカー |
品番 |
包装 |
PSC
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18-562
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50 UL
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※表示価格について
当社在庫 |
なし
|
納期目安 |
3週間程度
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保存温度 |
-20℃
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