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Western blot analysis of extracts of various cell lines, using MTHFD2 antibody (13-620) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human mammary cancer using MTHFD2 antibody (13-620) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using MTHFD2 antibody (13-620) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using MTHFD2 antibody (13-620) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using MTHFD2 antibody (13-620) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using MTHFD2 antibody (13-620) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using MTHFD2 antibody (13-620) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using MTHFD2 antibody (13-620) at dilution of 1:100. Blue: DAPI for nuclear staining.
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