別品名 |
MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma Growth Factor; Hepatocyte Stimulating Factor; Cytotoxic T-Cell Differentiation Factor
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種由来 |
Canine
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Accession No.(Gene/Protein) |
P41323
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タグ(タンパク質) |
N-terminal His Tag
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発現領域 |
a.a.18-207
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純度 |
>95%
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分子量 |
Predicted Molecular Mass(KD); 24.9kDa, Accurate Molecular Mass(KD); 25kDa
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形状 |
凍結乾燥品
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その他 |
[Buffer]20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
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※サムネイル画像をクリックすると拡大画像が表示されます。
Figure. Western Blot
Figure. SDS-PAGE
Figure. Cell proliferation of Raji cells after stimulated with IL6.
Figure. Cell proliferation of Raji cells after stimulated with IL6. Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. Interleukin 6 is secreted by T cells and macrophages to stimulate immune response and also plays a role in fighting infection. It supports the growth of B cells and is antagonistic to regulatory T cells. To test the effect of IL6 on cell proliferation, Raji cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with 1% serum standard 1640?which contains various concentrations of recombinant dog IL6. After incubated for 5 days,?cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of Raji cells after incubation with IL6 for 5 days observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant IL6 for 5 days. The result was shown in Figure 2. It was obvious that IL6 significantly increased cell viability of Raji cells. (A) Raji cells cultured in 1640, stimulated with 1ng/mL IL6 for 5 days; (B) Unstimulated Raji cells cultured in 1640 for 5 days.
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Figure. Western Blot
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メーカー |
品番 |
包装 |
WLS
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APA079CA01
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50 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
-70℃
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