Active Interleukin 2 (IL2)  

Figure. Western Blot; Sample: Recombinant IL2, Mouse.

Interleukin-2？(IL-2), the main lymphokine produced by activated CD4 T-helper lymphocytes, is a potent T-cell growth regulator. In addition to this activity, IL-2 was found to decrease the proliferation of 3T3 fibroblasts in time- and dose-dependent manner. Thus 3T3 cells were seeded overnight at a density of 5,000 cells/well, and treated with or without various concentrations of IL2 for 48h, then 3T3 cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37oC.<br/>The inhibitory effect of IL-2 on cell proliferation of 3T3 cells observed by inverted microscope was shown in Figure 1.

The dose-effect curve of IL2 was shown in Figure 2. It was obvious that IL2 significantly decreased cell growth of 3T3 cells. The ED50 for this effect is typically 9.876 to 13.35ng/mL.