適用 |
Immunoprecipitation
|
免疫動物 |
Mouse
|
抗体クラス |
IgG1
|
クローン |
OTI1C8
|
交差種 |
Streptococcus pyogenes
|
GENE ID |
109864281
|
Gene Symbol |
CAS9
|
添加剤 |
Carrier-free (BSA/glycerol-free)
|
その他 |
[locus ID]109864281
|
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Immunoprecipitation (IP) of Cas9 and Cas9-nickase by using mouse monoclonal anti-CAS9 antibodies TA811194 and TA811327. Mouse IgG control serves as the negative control. 293T cells were transfected with flag-tagged Cas9 overexpression plasmid, pCas-Guide (G) and pCas-Guide-nickase (N). Overexpression cell lysates were first precleared with ProteinG beads then incubated with beads crosslinked with anti-CAS9 antibody for overnight. The beads were then rinced with buffer and went through Western Blot analysis using anti-flag antibody (TA180144) (1:30).
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|
Immunoprecipitation (IP) of Cas9 and Cas9-nickase by using mouse monoclonal anti-CAS9 antibodies TA811194 and TA811327. Mouse IgG control serves as the negative control. 293T cells were transfected with flag-tagged Cas9 overexpression plasmid, pCas-Guide (G) and pCas-Guide-nickase (N). Overexpression cell lysates were first precleared with ProteinG beads then incubated with beads crosslinked with anti-CAS9 antibody for overnight. The beads were then rinced with buffer and went through Western Blot analysis using anti-flag antibody (TA180144) (1:30).
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メーカー |
品番 |
包装 |
ORG
|
CF811327
|
100 UG
|
※表示価格について
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