精製度 |
Purified
|
適用 |
Western Blot Enzyme Linked Immunosorbent Assay Immunoprecipitation
|
免疫動物 |
Mouse
|
抗体クラス |
IgG1
|
クローン |
11C2
|
交差種 |
Human Mouse
|
翻訳後修飾 |
リン酸化
|
GENE ID |
1956
|
Accession No.(Gene/Protein) |
NP_005219.2, P00533
|
Gene Symbol |
EGFR
|
その他 |
[locus ID]1956
|
※サムネイル画像をクリックすると拡大画像が表示されます。
EGFR Activation Serum starved MDA-MB 468 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab EGFR-11C2 (0.5 ug/ ml) for 1 h at RT and developed by ECL (exp. time: 30 sec). Lane 1: Control; Lane 2: 5 min EGF; Lane 3: 15 min EGF; Lane 4: 30 min EGF; Lane 5: 1h EGF; Lane 6; 2h EGF; Lane 7: 4h EGF; Lane 8: 8h EGF.
|
|
EGFR Activation Serum starved MDA-MB 468 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab EGFR-11C2 (0.5 ug/ ml) for 1 h at RT and developed by ECL (exp. time: 30 sec). Lane 1: Control; Lane 2: 5 min EGF; Lane 3: 15 min EGF; Lane 4: 30 min EGF; Lane 5: 1h EGF; Lane 6; 2h EGF; Lane 7: 4h EGF; Lane 8: 8h EGF.
|
|
|
メーカー |
品番 |
包装 |
ORG
|
AM00034PU-N
|
100 UG
|
※表示価格について
|