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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dibromo-tyrosine Monoclonal Antibody, Clone 6G3. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dibromo-tyrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 ﾂｵM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) Dibromo-tyrosine Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Western Blot analysis of 3, 5-Dibromotyrosine-BSA Conjugate showing detection of 67 kDa Dibromo-tyrosine protein using Mouse Anti-Dibromo-tyrosine Monoclonal Antibody, Clone 6G3. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: Nitrosylated-BSA. Lane 4: Dibromotyrosine-BSA. Load: 1 ﾂｵg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Dibromo-tyrosine Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Dibromo-tyrosine protein using Mouse Anti-Dibromo-tyrosine Monoclonal Antibody, Clone 6G3. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 ﾂｵg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Dibromo-tyrosine Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.
Dot Blot analysis using Mouse Anti-Dibromo-tyrosine Monoclonal Antibody, Clone 6G3. Tissue: 3, 5-Dibromotyrosine-BSA Conjugate. Species: Species Independent. Primary Antibody: Mouse Anti-Dibromo-tyrosine Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:4000 for 1 hour at RT. The quantities of protein spotted on each panel are as shown.
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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dibromo-tyrosine Monoclonal Antibody, Clone 6G3. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dibromo-tyrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 ﾂｵM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) Dibromo-tyrosine Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
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| 種由来 |
All Species
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| 標識物 |
ATTO 488
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| 精製度 |
Ig fraction - Protein G
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immuno Fluorescence
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| 免疫動物 |
Mouse
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| 抗体クラス |
IgG1
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| クローン |
6G3
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| 交差種 |
All Species
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| メーカー |
品番 |
包装 |
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LSP
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LS-C820948-100
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約1ヶ月
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| 保存温度 |
-２０℃
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