別品名 |
Rabbit Anti-CD68 Antibody, Rabbit Anti-Macrosialin Antibody, Gp110, CD68, CD68 Molecule, CD68 Antigen, Scavenger Receptor Class D Member, Macrophage Antigen CD68, SCARD1, LAMP4, Macrosialin
|
抗原部位 |
C-Terminus
|
種由来 |
Mouse
|
標識物 |
Unlabeled
|
精製度 |
Affinity Purified
|
適用 |
Western Blot Enzyme Linked Immunosorbent Assay
|
免疫動物 |
Rabbit
|
交差種 |
Mouse
|
GENE ID |
12514
|
Accession No.(Gene/Protein) |
NP_001277987, P31996
|
Gene Symbol |
CD68
|
形状 |
液状
|
その他 |
[Buffer]0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
|
参考文献 |
[Pub Med ID]27730347
|
[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
|
※サムネイル画像をクリックすると拡大画像が表示されます。
ELISA results of purified Rabbit Anti CD68 Antibody tested against BSA conjugated CD68 immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5μg/ml and the X axis represents the Log10 of a 3 fold dilution. This titration is a 4 parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using ELISA Microwell Blocking Buffer (p/n MB 064 1000), Goat Anti Rabbit IgG HRP conjugated (p/n 611 103 122), and TMBE 1000.
Western Blot of Rabbit Anti-CD68 Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: HeLa + Rec. CD68 (Baculo expressed) protein (10μg/0.05μg) [+]. Lane 3: HeLa + Rec. CD68 (Baculo expressed) protein (10μg/0.02μg) [+]. Lane 4: HeLa + Rec. CD68 (Baculo expressed) protein (10μg/0.01μg) [+]. Lane 5: HeLa whole cell lysate (p/n W09-000-364) (10μg) [-]. Primary Antibody: Anti-CD68 at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:70,000 for 60 mins at RT. Block: BlockOut Buffer (p/n MB-073). Expected MW: ~60-70kDa recombinant CD68. Observed MW: ~75kDa.
Western Blot of Rabbit Anti-CD68 Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: Mouse Liver tissue lysate (p/n W10-000-T020) [+]. Lane 3: Mouse Lung tissue lysate (p/n W10-000-MQ1) [+]. Lane 4: Mouse Spleen tissue lysate (p/n W10-000-T029) [+]. Lane 5: Mouse Heart tissue lysate (p/n W10-000-T014) [low]. Lane 6: Mouse Testis tissue lysate (p/n W10-000-GZ2) [low]. Lane 7: Mouse Kidney tissue lysate (p/n W10-000-T017) [low]. Lane 8: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Primary Antibody: Anti-CD68 at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:70,000 for 60 mins at RT. Block: BlockOut Buffer (p/n MB-073). Expected MW: ~34kDa with glycosylations. Observed MW: ~34kDa.
|
|
ELISA results of purified Rabbit Anti CD68 Antibody tested against BSA conjugated CD68 immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5μg/ml and the X axis represents the Log10 of a 3 fold dilution. This titration is a 4 parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using ELISA Microwell Blocking Buffer (p/n MB 064 1000), Goat Anti Rabbit IgG HRP conjugated (p/n 611 103 122), and TMBE 1000.
|
|
|
メーカー |
品番 |
包装 |
RKL
|
600-401-MP2
|
100 UG
|
※表示価格について
当社在庫 |
なし
|
納期目安 |
約10日
|
保存温度 |
-20℃
|
|